Anticorps Polyclonal de lapin anti-SARS-CoV-2 Membrane Glycoprotein

SARS-CoV-2 Membrane Glycoprotein Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 28882-1-AP

Synonymes

2019-nCOV membrane glycoprotein, M protein, membrane glycoprotein

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Galerie de données de validation

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  ELISA experiment of Recombinant protein using 28882-1-AP

  COVID-19 M Protein Antibody (28882-1-AP) tested by ELISA. COVID-19 M Protein(101-222aa ) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of COVID-19 M Protein Antibody (28882-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

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Applications testées

• Résultats positifs en ELISA: Protéine recombinante,

Dilution recommandée

• Test immuno-enzymatique (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

28882-1-AP cible SARS-CoV-2 Membrane Glycoprotein dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARS-CoV-2 Membrane Glycoprotein Protéine recombinante Ag30691
• Nom complet: COVID-19 M Protein
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: SARS-CoV-2 Membrane Protein
• Identification du gène (NCBI): 43740571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In the coronaviruses, the membrane (M) protein was reported to be the most abundant viral protein expressed during infection and a key protein in the assembly of both naked and enveloped virus particles (PMID: 11967315). The M glycoprotein is conserved across the β-coronaviruses. The multiple sequence alignment shows a remarkable similarity (98% identity) among the Sars-CoV-2 M variants and the sequences from Bat and Pangolin isolates (PMID:32596311). The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior (PMID: 15626342).

Publications

Species	Application	Title
	WB,IF	bioRxiv; Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus; Authors - Qiwang Xiang
		J Virol; Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation; Authors - Zhaohuan Wang

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