Anticorps Polyclonal de lapin anti-SARS-CoV-2 Nucleocapsid Phosphoprotein

SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 28769-1-AP

Synonymes

N protein, nucleocapsid phosphoprotein

---

Galerie de données de validation

• 

  WB analysis using 28769-1-AP

  SARS-CoV-2 Nucleocapsid Phosphoprotein with the domain 1-419aa expressed in HEK-293 cells were subjected to SDS PAGE followed by western blot with 28769-1-AP (2019-nCOV nucleocapsid phosphoprotein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  ELISA experiment of Recombinant protein using 28769-1-AP

  SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP) tested by ELISA.SARS-CoV-2 Nucleocapsid Phosphoprotein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

---

Applications testées

• Résultats positifs en WB: Protéine recombinante,
• Résultats positifs en ELISA: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Test immuno-enzymatique (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

28769-1-AP cible SARS-CoV-2 Nucleocapsid Phosphoprotein dans les applications de WB, ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARS-CoV-2 Nucleocapsid Phosphoprotein Protéine recombinante Ag30676
• Nom complet: COVID-19 N Protein
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: COVID-19 N Protein
• Identification du gène (NCBI): 43740575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity.(PMID: 32416961, PMID: 32235387)

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; SARS-CoV-2 NSP5 and N protein counteract the RIG-I signaling pathway by suppressing the formation of stress granules.; Authors - Yi Zheng
human	WB	Antiviral Res; RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5ʹ-untranslated region; Authors - Yongxuan Yao
human	WB	Virol J; LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19; Authors - Xinyi Kong

{{ptg:RelatedPrimaryAntibodies}}