Anticorps Monoclonal anti-SARS-CoV-2 Nucleocapsid Phosphoprotein

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Virus et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1B3C3

N° de cat : 67666-1-Ig

Synonymes

nucleocapsid phosphoprotein, 1B3C3, sars cov 2

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Galerie de données de validation

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  WB analysis using 67666-1-Ig

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration.

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  ELISA experiment of Recombinant protein using 67666-1-Ig

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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  ELISA experiment of Recombinant protein using 67666-1-Ig

  Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

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Applications testées

• Résultats positifs en WB: Ag30676,
• Résultats positifs en ELISA: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Test immuno-enzymatique (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67666-1-Ig cible SARS-CoV-2 Nucleocapsid Phosphoprotein dans les applications de WB, ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SARS-CoV-2 Nucleocapsid Phosphoprotein Protéine recombinante Ag30676
• Nom complet: COVID-19 N Protein
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: COVID-19 N Protein
• Identification du gène (NCBI): 43740575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.

Publications

Species	Application	Title
mouse		Biosens Bioelectron; Electronic field effect detection of SARS-CoV-2 N-protein before the onset of symptoms.; Authors - I Novodchuk
		Viruses; Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins.; Authors - Marina Pribanić Matešić
		J Virol; Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation; Authors - Zhaohuan Wang

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