Anticorps Recombinant de lapin anti-SARS-CoV-2 Nucleocapsid Phosphoprotein

SARS-CoV-2 Nucleocapsid Phosphoprotein Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante, Virus et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

8C20

N° de cat : 80027-1-RR

Synonymes

nucleocapsid phosphoprotein, sars cov 2, 8C20

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Galerie de données de validation

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  WB analysis of Recombinant protein using 80027-1-RR

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

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  WB analysis of Recombinant protein using 80027-1-RR

  Eukaryotic expressed SARS-CoV-2 Nucleocapsid Phosphoprotein was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

• 

  ELISA experiment of Recombinant protein using 80027-1-RR

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 80026-1-RR and 80027-1-RR respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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  ELISA experiment of Recombinant protein using 80027-1-RR

  Sandwich ELISA was carried out by coating 80027-1-RR at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated80026-1-RR was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

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  Affinity and Kinetic Characterization of 80027-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80027-1-RR against SARS-CoV-2 Nucleocapsid Phosphoprotein were performed. The affinity constant is 1.02 nM.

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Applications testées

• Résultats positifs en WB: Phosphoprotéine nucléocapside eucaryote, Protéine recombinante
• Résultats positifs en ELISA: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Test immuno-enzymatique (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

80027-1-RR cible SARS-CoV-2 Nucleocapsid Phosphoprotein dans les applications de WB, ELISA et montre une réactivité avec des échantillons Protéine recombinante, Virus

• Réactivité: Protéine recombinante, Virus
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SARS-CoV-2 Nucleocapsid Phosphoprotein Protéine recombinante Ag30676
• Nom complet: COVID-19 N Protein
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: COVID-19 N Protein
• Identification du gène (NCBI): 43740575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387). A sandwich ELISA for COVID-19 N Protein can be assembled by using 80027-1-RR as capture antibody and conjugated 80026-1-RR for detection.

Publications

Species	Application	Title
human	WB	Virol Sin; The SARS-CoV-2 3CL protease inhibits pyroptosis through the cleavage of gasdermin D; Authors - Yecheng Zhang

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