Anticorps Monoclonal anti-4-Hydroxynonenal

4-Hydroxynonenal Monoclonal Antibody for ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

4-hydroxynonenal, chemical compound

Applications

ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C1D10

N° de cat : 68538-1-PBS

Synonymes

Cas. 75899-68-2, C9H16O2, 4-Hydroxy-2-nonenal, 4-HNE

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Galerie de données de validation

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  ELISA experiment of 4-Hydroxynonenal using 68538-1-Ig (same clone as 68538-1-PBS)

  Indirect ELISA was performed by coating BSA conjugated 4-Hydroxynonenal (4-HNE) at ~20 ng/well (by 4-HNE amount), followed by blocking with 1% BSA. Serial diluted 4-Hydroxynonenal antibody 68538-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of 4-Hydroxynonenal was mixed in 5 ng/mL primary antibody (bottom X-left Y, red curve). This data was developed using the same antibody clone with 68538-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68538-1-PBS cible 4-Hydroxynonenal dans les applications de ELISA, Indirect ELISA et montre une réactivité avec des échantillons 4-hydroxynonenal, chemical compound

• Réactivité: 4-hydroxynonenal, chemical compound
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTG
• Nom complet: 4-Hydroxynonenal
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

4-Hydroxynonenal is a uremic toxin. Uremic toxins can be subdivided into three major groups based upon their chemical and physical characteristics: 1) small, water-soluble, non-protein-bound compounds, such as urea; 2) small, lipid-soluble and/or protein-bound compounds, such as the phenols and 3) larger so-called middle-molecules, such as beta2-microglobulin. 4-Hydroxynonenal (4-HNE) is a major aldehydic product of ω-6-unsaturated fatty acid peroxidation. It is considered a lipid peroxidation specific marker. 4-HNE has been found to induce differentiation and inhibit proliferation of HL-60 human leukemic cells. It has also been found to induce murine alveolar macrophage cell death. 4-HNE has been shown to inhibit State 3 respiration, causing a transient cytosolic Ca2+ increase. In addition, it irreversibly inhibits Na+-K+-ATPase activity.

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