- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-4EBP1
4EBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Mouse / IgG2b
Réactivité testée
Humain, porc
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
CloneNo.
2C3F3
N° de cat : 60246-1-PBS
Synonymes
Galerie de données de validation
Informations sur le produit
60246-1-PBS cible 4EBP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc
Réactivité | Humain, porc |
Hôte / Isotype | Mouse / IgG2b |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | 4EBP1 Protéine recombinante Ag18985 |
Nom complet | eukaryotic translation initiation factor 4E binding protein 1 |
Masse moléculaire calculée | 118 aa, 12 kDa |
Poids moléculaire observé | 15-24 kDa |
Numéro d’acquisition GenBank | BC004459 |
Symbole du gène | EIF4EBP1 |
Identification du gène (NCBI) | 1978 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS only |
Conditions de stockage | Store at -80°C. 20ul contiennent 0,1% de BSA. |
Informations générales
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)