Anticorps Recombinant de lapin anti-Phospho-4EBP1 (Thr37)

Phospho-4EBP1 (Thr37) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240535G3

N° de cat : 81812-4-PBS

Synonymes

4EBP1 (Thr37), 4E-BP1 (Thr37), p-4EBP1, p-4E-BP1, p-4EBP1 (Thr37)

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Galerie de données de validation

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  WB analysis using 81812-4-RR (same clone as 81812-4-PBS)

  Non-treated HEK-293T cells, phosphatase inhibitor treated HEK-293T cells and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81812-4-RR (Phospho-4EBP1 (Thr37) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81812-4-PBS in a different storage buffer formulation.

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Informations sur le produit

81812-4-PBS cible Phospho-4EBP1 (Thr37) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 4E binding protein 1
• Masse moléculaire calculée: 118 aa, 12 kDa
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC004459
• Symbole du gène: EIF4EBP1
• Identification du gène (NCBI): 1978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)

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