Anticorps Recombinant de lapin anti-4EBP1

• Phare
• Validé par KD/KO

4EBP1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241055E7

N° de cat : 83890-5-RR

Synonymes

EIF4EBP1, 241055E7, 4E BP1, 4E-BP1, BP 1

---

Galerie de données de validation

• 

  WB analysis using 83890-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83890-5-RR (4EBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 83890-5-RR

  WB result of 4EBP1 antibody (83890-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-4EBP1 transfected K-562 cells.

• 

  FC experiment of HeLa using 83890-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug 4EBP1 Recombinant antibody (83890-5-RR, Clone:241055E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of HEK-293 using 83890-5-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug 4EBP1 Recombinant antibody (83890-5-RR, Clone:241055E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 83890-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83890-5-RR against Human 4EBP1 were performed. The affinity constant is 0.752 nM.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HeLa, cellules HepG2, cellules HL-60, cellules K-562
• Résultats positifs en FC (Intra): cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83890-5-RR cible 4EBP1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: 4EBP1 Protéine recombinante Ag5056
• Nom complet: eukaryotic translation initiation factor 4E binding protein 1
• Masse moléculaire calculée: 118 aa, 12 kDa
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC004459
• Symbole du gène: EIF4EBP1
• Identification du gène (NCBI): 1978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)

{{ptg:RelatedPrimaryAntibodies}}