Anticorps Polyclonal de lapin anti-5 Lipoxygenase

• Phare
• Validé par KD/KO

5 Lipoxygenase Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10021-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10021-1-Ig (same clone as 10021-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10021-1-Ig (same clone as 10021-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 10021-1-Ig (same clone as 10021-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 10021-1-Ig (same clone as 10021-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10021-1-Ig (same clone as 10021-1-PBS)

  Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10021-1-PBS cible 5 Lipoxygenase dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: arachidonate 5-lipoxygenase
• Masse moléculaire calculée: 674 aa, 78 kDa
• Poids moléculaire observé: 70-78 kDa
• Numéro d’acquisition GenBank: BC130332
• Symbole du gène: 5 Lipoxygenase
• Identification du gène (NCBI): 240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

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