Anticorps Monoclonal anti-5 Lipoxygenase

• Phare
• Validé par KD/KO

5 Lipoxygenase Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

3B1B7

N° de cat : 66326-1-PBS

Synonymes

ALOX5, 3B1B7, 5 LO, 5 LOX, 5-lipoxygenase

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Galerie de données de validation

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  WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

  WB result of 5 Lipoxygenase antibody (66326-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-5 Lipoxygenase transfected THP-1 cells. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66326-1-Ig (same clone as 66326-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66326-1-PBS cible 5 Lipoxygenase dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: 5 Lipoxygenase Protéine recombinante Ag18168
• Nom complet: arachidonate 5-lipoxygenase
• Masse moléculaire calculée: 674 aa, 78 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC130332
• Symbole du gène: 5 Lipoxygenase
• Identification du gène (NCBI): 240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

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