Anticorps Recombinant de lapin anti-5'-Nucleotidase/CD73

5'-Nucleotidase/CD73 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241541B10

N° de cat : 84434-7-PBS

Synonymes

EC:3.1.3.99, CD73, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP01279-4

  Sandwich ELISA standard curve of MP01279-4, Mouse CD73 Recombinant Matched Antibody Pair - PBS only. 84434-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1389. 84434-7-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP01279-4

  The mean CD73 concentration was determined to be 7.68 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

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  Sample test of MP01279-4

  Mouse liver and brain tissue were rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C. Homogenates were thawed and centrifuged for 5 minutes at 5000 x g. Homogenates were assayed for mouse CD73 and measured 23.05 ng/mL based on a 3.3 mg/mL homogenate load, and 4.37 ng/mL based on a 2.2 mg/mL homogenate load, respectively.

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Informations sur le produit

84434-7-PBS cible 5'-Nucleotidase/CD73 dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: 5' nucleotidase, ecto
• Masse moléculaire calculée: 64kDa
• Numéro d’acquisition GenBank: NM_011851.4
• Symbole du gène: Nt5e
• Identification du gène (NCBI): 23959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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