Anticorps Polyclonal de lapin anti-6*His, His-tag
6*His, His-tag Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Protéine recombinante et plus (2)
Applications
WB, IHC, IF, IP, CoIP, ChIP, ELISA
Conjugaison
Non conjugué
N° de cat : 10001-0-AP
Synonymes
"6*His, His-tag Antibodies" Comparison
View side-by-side comparison of 6*His, His-tag antibodies from other vendors to find the one that best suits your research needs.
Applications testées
| Résultats positifs en WB | Protéine recombinante |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 93 publications below |
| IHC | See 1 publications below |
| IF | See 10 publications below |
| IP | See 17 publications below |
| CoIP | See 3 publications below |
| ChIP | See 1 publications below |
Informations sur le produit
10001-0-AP cible 6*His, His-tag dans les applications de WB, IHC, IF, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Protéine recombinante |
| Nom complet | 6*His, His-tag |
| Masse moléculaire calculée | 1 kDa |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for 6*His, His-tag antibody 10001-0-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. | ||
Cell Death Differ SLC15A3 plays a crucial role in pulmonary fibrosis by regulating macrophage oxidative stress | ||
Nat Commun Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery. | ||
Nat Commun Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Brogan (Verified Customer) (11-09-2022) | Works very well, will buy again
|
FH Jean-Baptiste (Verified Customer) (04-21-2022) | Tested in Western blot for analysing the production in bacteria and the purification of a recombinant his-tagged protein. Worked quite well, with few unspecific bands.
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