Anticorps Recombinant de lapin anti-6*his,His tag

6*his,His tag Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241261A7

N° de cat : 84814-1-PBS

Synonymes

6*His, His-Tag, 6*His, His Tag, His Tag, 6XHis, 6histag

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Galerie de données de validation

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  WB analysis using 84814-1-RR (same clone as 84814-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84814-1-RR (6*his,His tag antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84814-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 84814-1-RR (same clone as 84814-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 6*his,His tag antibody (84814-1-RR, Clone: 241261A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84814-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 84814-1-RR (same clone as 84814-1-PBS)

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.25 ug 6*his,His tag Recombinant antibody (84814-1-RR, Clone:241261A7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84814-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84814-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with C-terminal 6*His Tag were performed. The affinity constant is 26.1 nM.

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  Affinity and Kinetic Characterization of 84814-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with N-terminal 6*His Tag were performed. The affinity constant is 1.69 nM.

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Informations sur le produit

84814-1-PBS cible 6*his,His tag dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: 6*his,His tag
• Masse moléculaire calculée: 1 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.

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