Anticorps Polyclonal de lapin anti-A2BP1

• Phare
• Validé par KD/KO

A2BP1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 22647-1-AP

Synonymes

FOX1, RBFOX1, HRNBP1, Hexaribonucleotide-binding protein 1, Fox-1 homolog A

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Galerie de données de validation

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  WB analysis using 22647-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22647-1-AP (A2BP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22647-1-AP

  IP result of anti-A2BP1 (IP:22647-1-AP, 4ug; Detection:22647-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 22647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 22647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse brain using 22647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 22647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 22647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cerveau de souris, foie de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

22647-1-AP cible A2BP1 dans les applications de WB, IHC, IF-P, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: A2BP1 Protéine recombinante Ag18527
• Nom complet: ataxin 2-binding protein 1
• Masse moléculaire calculée: 395 aa, 42 kDa
• Poids moléculaire observé: 45-70 kDa
• Numéro d’acquisition GenBank: BC113691
• Symbole du gène: A2BP1
• Identification du gène (NCBI): 54715
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

A2BP1, also named as FOX1 and HRNBP1, contains one RRM (RNA recognition motif) domain. A2BP1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. A2BP1 and ataxin-2 are both localized in the trans-Golgi network. This is a rabbit polyclonal antibody raised against the N terminus of human A2BP1.A2BP1 has two mouse isoforms (A2BP1-A016 and -A030) exogenously expressed in COS7 cells showed a molecular mass of 65 kDa, although the calculated molecular masses of A016 and A030 are 40 and 42 kDa, respectively in the course of cell biological study.

Publications

Species	Application	Title
human	WB,RIP	Mol Ther Oncolytics; RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.; Authors - Shuyuan Shen ; KD Validated
human	IF	MedComm (2020); Single-cell transcriptomics reveal the remodeling landscape of bladder in patients with obstruction-induced detrusor underactivity; Authors - Jiawei Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Karthik (Verified Customer) (09-03-2025)

Cortical neurons permeabilized with 0.25% triton x for 10 minutes and labeled with 1:250 dilution of RBFOX1 ab for 2 hours at room temperature.

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Mouse cortical neurons

A2BP1 Antibody Immunofluorescence,Cell culture validation (1:250 dilution) in Mouse cortical neurons (Cat no:22647-1-AP)

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