Anticorps Polyclonal de lapin anti-A4GNT

A4GNT Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 22670-1-AP

Synonymes

EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase

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Galerie de données de validation

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  WB analysis using 22670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat stomach using 22670-1-AP

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 22670-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 22670-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 22670-1-AP (A4GNT antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat stomach using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse stomach using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse stomach using 22670-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules L02, tissu d'estomac de rat, tissu d'estomac de souris, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du pancréas humain, tissu d'estomac de rat, tissu d'estomac de souris, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22670-1-AP cible A4GNT dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: A4GNT Protéine recombinante Ag18432
• Nom complet: alpha-1,4-N-acetylglucosaminyltransferase
• Masse moléculaire calculée: 340 aa, 39 kDa
• Poids moléculaire observé: 34-37 kDa
• Numéro d’acquisition GenBank: BC119639
• Symbole du gène: A4GNT
• Identification du gène (NCBI): 51146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.

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