Anticorps Polyclonal de lapin anti-A4GNT

A4GNT Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22670-1-PBS

Synonymes

EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase

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Galerie de données de validation

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  WB analysis using 22670-1-AP (same clone as 22670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 22670-1-AP (same clone as 22670-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 22670-1-AP (A4GNT antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22670-1-PBS cible A4GNT dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: A4GNT Protéine recombinante Ag18432
• Nom complet: alpha-1,4-N-acetylglucosaminyltransferase
• Masse moléculaire calculée: 340 aa, 39 kDa
• Poids moléculaire observé: 34-37 kDa
• Numéro d’acquisition GenBank: BC119639
• Symbole du gène: A4GNT
• Identification du gène (NCBI): 51146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.

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