Anticorps Polyclonal de lapin anti-AACS

• Phare
• Validé par KD/KO

AACS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13815-1-PBS

Synonymes

Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1

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Galerie de données de validation

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  WB analysis of mouse heart using 13815-1-AP (same clone as 13815-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 13815-1-AP (same clone as 13815-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 13815-1-AP (same clone as 13815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13815-1-AP (AACS antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13815-1-AP (same clone as 13815-1-PBS)

  Immunofluorescent analysis of MCF-7 cells using 13815-1-AP (AACS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13815-1-PBS cible AACS dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AACS Protéine recombinante Ag4899
• Nom complet: acetoacetyl-CoA synthetase
• Masse moléculaire calculée: 672 aa, 75 kDa
• Poids moléculaire observé: 67 kDa, 30 kDa
• Numéro d’acquisition GenBank: BC040490
• Symbole du gène: AACS
• Identification du gène (NCBI): 65985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.

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