Anticorps Polyclonal de lapin anti-AASDHPPT

AASDHPPT Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 11244-1-AP

Synonymes

CGI-80, CGI 80, Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, AASD PPT

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Galerie de données de validation

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  WB analysis of human brain using 11244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 11244-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AASDHPPT antibody (11244-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

11244-1-AP cible AASDHPPT dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AASDHPPT Protéine recombinante Ag1763
• Nom complet: aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC016728
• Symbole du gène: AASDHPPT
• Identification du gène (NCBI): 60496
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AASDHPPT, also known as L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, is required for mitochondrial respiration and oxidative metabolism via the mitochondrial fatty acid synthesis (mtFAS) pathway. AASDHPPT catalyzes the post-translational modification of target proteins by phosphopantetheine and transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, including the acyl carrier domain of FASN.

Publications

Species	Application	Title
mouse	WB	bioRxiv; Mitochondrial Phosphopantetheinylation is Required for Oxidative Function; Authors - Pieter R Norden

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