Anticorps Polyclonal de lapin anti-AATF

• Phare
• Validé par KD/KO

AATF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10282-1-PBS

Synonymes

Apoptosis-antagonizing transcription factor, CHE 1, CHE1, DED, HSPC277

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Galerie de données de validation

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  WB analysis using 10282-1-AP (same clone as 10282-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10282-1-AP (same clone as 10282-1-PBS)

  WB result of AATF antibody (10282-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AATF transfected HepG2 cells. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10282-1-AP (same clone as 10282-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 10282-1-AP (same clone as 10282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10282-1-AP (AATF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10282-1-AP (same clone as 10282-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using AATF antibody (10282-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10282-1-PBS cible AATF dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AATF Protéine recombinante Ag0195
• Nom complet: apoptosis antagonizing transcription factor
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 80-95 kDa
• Numéro d’acquisition GenBank: BC000591
• Symbole du gène: AATF
• Identification du gène (NCBI): 26574
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex.

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