Anticorps Polyclonal de lapin anti-ABCA1

• Phare
• Validé par KD/KO

ABCA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 26564-1-AP

Synonymes

ABC1, ABC-1, ATP-binding cassette 1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1

---

Galerie de données de validation

• 

  WB analysis using 26564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26564-1-AP (ABCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26564-1-AP (ABCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 26564-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26564-1-AP (ABCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de cervelet de souris
• Résultats positifs en IHC: tissu pulmonaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

26564-1-AP cible ABCA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABCA1 Protéine recombinante Ag24118
• Nom complet: ATP-binding cassette, sub-family A (ABC1), member 1
• Masse moléculaire calculée: 2261 aa, 254 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC141816
• Symbole du gène: ABCA1
• Identification du gène (NCBI): 19
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABCA, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway.

Publications

Species	Application	Title
mouse	WB	Clin Transl Med; Tumour-associated macrophage-derived DOCK7-enriched extracellular vesicles drive tumour metastasis in colorectal cancer via the RAC1/ABCA1 axis; Authors - Weiwei Chen ; KD Validated
mouse,human	WB,IHC	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu
mouse	WB,IHC	Atherosclerosis; Flagellar hook protein FlgE promotes macrophage activation and atherosclerosis by targeting ATP5B; Authors - Yuanyuan Li
human	IF	Front Cardiovasc Med; miR-3529-3p/ABCA1 axis regulates smooth muscle cell homeostasis by enhancing inflammation via JAK2/STAT3 pathway; Authors - Tingyu Wang
human	IHC	Biofactors; Investigating the dysregulation of genes associated with glucose and lipid metabolism in gastric cancer and their influence on immunity and prognosis; Authors - Yan Li
mouse	WB	Drug Des Devel Ther; Baolier Capsule's Secret Weapon: Piperine Boosts Cholesterol Excretion to Combat Atherosclerosis; Authors - Mengqiu Wei

{{ptg:RelatedPrimaryAntibodies}}