Anticorps Polyclonal de lapin anti-ABCB8

• Phare
• Validé par KD/KO

ABCB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 27158-1-PBS

Synonymes

ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1

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Galerie de données de validation

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  WB analysis of MCF-7 using 27158-1-AP (same clone as 27158-1-PBS)

  WB result of ABCB8 antibody (27158-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCB8 transfected MCF-7 cells. This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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  WB analysis using 27158-1-AP (same clone as 27158-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27158-1-AP (ABCB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 27158-1-AP (same clone as 27158-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ABCB8 antibody (27158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27158-1-PBS cible ABCB8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABCB8 Protéine recombinante Ag25935
• Nom complet: ATP-binding cassette, sub-family B (MDR/TAP), member 8
• Masse moléculaire calculée: 735 aa, 80 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC141814
• Symbole du gène: ABCB8
• Identification du gène (NCBI): 11194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ABCB8 (ATP-binding cassette sub-family B member 8) is a protein located in the mitochondrial inner membrane and belongs to the ATP-binding cassette (ABC) transporter superfamily. It plays a crucial role in maintaining mitochondrial iron homeostasis and the maturation of cellular iron-sulfur cluster proteins. ABCB8 is essential for normal cardiac function by promoting the export of iron from mitochondria. Additionally, ABCB8 forms a complex with the mitochondrial KATP channel, protecting cells from oxidative stress damage. The loss of function or abnormal expression of ABCB8 is associated with various diseases, including cardiomyopathy and iron metabolism disorders.

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