Anticorps Monoclonal anti-MRP1

MRP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(19)

CloneNo.

1G4A2

N° de cat : 67228-1-Ig

Synonymes

ABCC1, 1G4A2, MRP1/ABCC1

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Galerie de données de validation

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  WB analysis using 67228-1-Ig

  Non-boil and boiled HepG2 and A549 cell lysates were subjected to SDS PAGE followed by western blot with 67228-1-Ig (ABCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Left panel was exposed for 20 sec. and right panel was exposed for 1 min.

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  IF Staining of A549 using 67228-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MRP1 antibody (67228-1-Ig, Clone: 1G4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2 non bouillies, unboiled A549 cells
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

67228-1-Ig cible MRP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MRP1 Protéine recombinante Ag27048
• Nom complet: ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• Masse moléculaire calculée: 172 kDa
• Poids moléculaire observé: 180-190 kDa
• Numéro d’acquisition GenBank: NM_004996
• Symbole du gène: MRP1
• Identification du gène (NCBI): 4363
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Multidrug resistant-associated protein 1 (MRP1; ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.

Publications

Species	Application	Title
human	WB	Environ Pollut; Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium.; Authors - Jianqiang Zhu
human	WB	Cancers (Basel); NCAPG2 Maintains Cancer Stemness and Promotes Erlotinib Resistance in Lung Adenocarcinoma; Authors - Shiyao Jiang
human	WB	Commun Chem; Exploring bat-inspired cyclic tryptophan diketopiperazines as ABCB1 Inhibitors; Authors - Javier Yu Peng Koh ; KD Validated
human	WB	Front Cell Dev Biol; Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.; Authors - Wanshuang Cao
human	WB	Transl Oncol; Pan-cancer analysis of ABCC1 as a potential prognostic and immunological biomarker; Authors - Tiantian Wang
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng

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