Anticorps Recombinant de lapin anti-ABCD1

• Phare
• Validé par KD/KO

ABCD1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230226E12

N° de cat : 83038-4-PBS

Synonymes

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

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Galerie de données de validation

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  WB analysis of HeLa using 83038-4-RR (same clone as 83038-4-PBS)

  WB result of ABCD1 antibody (83038-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCD1 transfected HeLa cells. This data was developed using the same antibody clone with 83038-4-PBS in a different storage buffer formulation.

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  WB analysis using 83038-4-RR (same clone as 83038-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83038-4-RR (ABCD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83038-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83038-4-RR (same clone as 83038-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ABCD1 antibody (83038-4-RR, Clone: 230226E12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83038-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83038-4-RR (same clone as 83038-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ABCD1 Recombinant antibody (83038-4-RR, Clone:230226E12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83038-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83038-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83038-4-RR against Human ABCD1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83038-4-PBS cible ABCD1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ABCD1 Protéine recombinante Ag0453
• Nom complet: ATP-binding cassette, sub-family D (ALD), member 1
• Masse moléculaire calculée: 745 aa, 83 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC025358
• Symbole du gène: ABCD1
• Identification du gène (NCBI): 215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

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