Anticorps Monoclonal anti-ABCD3

ABCD3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1G7G5

Avis (1)

N° de cat : 66697-1-Ig

Synonymes

ABC43, ABCD3, PMP70, PXMP1

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Galerie de données de validation

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  WB analysis using 66697-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66697-1-Ig (ABCD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Caco-2, cellules HepG2, cellules HT-29, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

66697-1-Ig cible ABCD3 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ABCD3 Protéine recombinante Ag24760
• Nom complet: ATP-binding cassette, sub-family D (ALD), member 3
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC068509
• Symbole du gène: ABCD3
• Identification du gène (NCBI): 5825
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	IF	Hypertens Res; Metabolic phenotypes and fatty acid profiles associated with histopathology of primary aldosteronism; Authors - Yuhong Yang
human	WB	Life Sci Alliance; Non-canonical activation of the ER stress sensor ATF6 by Legionella pneumophila effectors; Authors - Nnejiuwa U Ibe
human	WB	Haematologica; Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.; Authors - Nunziata Maio
human	WB	bioRxiv; The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy; Authors - Iris D Montes
mouse	WB	Chin J Nat Med; Activation of LONP1 by 84-B10 alleviates aristolochic acid nephropathy via re-establishing mitochondrial and peroxisomal homeostasis; Authors - Xinyue Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PAULA (Verified Customer) (07-12-2023)

I tested this ABCD3 antibody at two dilutions on THP1 cells (20ug cell lysate) and I saw a nice band around 70 kDa, although some non-specific bands were also detected. Dilution 1:1000 showed similar results to 1:500 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000, 1:500
• Cell Tissue Type: THP1 cell line

ABCD3 Antibody Western Blot validation (1:1000, 1:500 dilution) in THP1 cell line (Cat no:66697-1-Ig)

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