Anticorps Polyclonal de lapin anti-ABCG5

ABCG5 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 27722-1-AP

Synonymes

ABCG5, Sterolin 1, STSL

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Galerie de données de validation

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  WB analysis using 27722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 27722-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27722-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 27722-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27722-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27722-1-AP (ABCG5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27722-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27722-1-AP (ABCG5 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HepG2, tissu de côlon de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 24 publications below

Informations sur le produit

27722-1-AP cible ABCG5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABCG5 Protéine recombinante Ag26844
• Nom complet: ATP-binding cassette, sub-family G (WHITE), member 5
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 68 kDa~72 kDa
• Numéro d’acquisition GenBank: NM_022436
• Symbole du gène: ABCG5
• Identification du gène (NCBI): 64240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Diabetes; Long Noncoding RNA lncRHL Regulates Hepatic VLDL Secretion by Modulating hnRNPU/ BMAL1/MTTP Axis.; Authors - Xuan Shen
human	WB	Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
human	WB	Mol Ther Nucleic Acids; HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.; Authors - Zhi Zhang
human	WB	J Cell Mol Med; The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα.; Authors - Ting Li
rat	WB	Front Nutr; Preparation of microgel co-loaded with nuciferine and epigallocatechin-3-gallate for the regulation of lipid metabolism; Authors - Shengnan Zhu
mouse,human	WB	Biochem Pharmacol; Resveratrol enhances trans-intestinal cholesterol excretion through selective activation of intestinal liver X receptor alpha.; Authors - Juan Pang