Anticorps Monoclonal anti-ABHD5

ABHD5 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B12C2

N° de cat : 67779-1-PBS

Synonymes

ABHD5, CDS, CGI58, IECN2, NCIE2

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Galerie de données de validation

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  WB analysis using 67779-1-Ig (same clone as 67779-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67779-1-Ig (ABHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67779-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67779-1-Ig (same clone as 67779-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ABHD5 antibody (67779-1-Ig, Clone: 2B12C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67779-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67779-1-PBS cible ABHD5 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ABHD5 Protéine recombinante Ag30256
• Nom complet: abhydrolase domain containing 5
• Masse moléculaire calculée: 349 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC021958
• Symbole du gène: ABHD5
• Identification du gène (NCBI): 51099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

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