Anticorps Monoclonal anti-ABL1

ABL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E7A5

N° de cat : 68254-1-PBS

Synonymes

c ABL, JTK7, EC:2.7.10.2, ABL, Abelson tyrosine-protein kinase 1

---

Galerie de données de validation

• 

  WB analysis using 68254-1-Ig (same clone as 68254-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68254-1-Ig (ABL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68254-1-Ig (same clone as 68254-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 68254-1-Ig (same clone as 68254-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 68254-1-Ig (same clone as 68254-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABL1 antibody (68254-1-Ig, Clone: 1E7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 68254-1-Ig (same clone as 68254-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ABL1 (68254-1-Ig, Clone:1E7A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68254-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68254-1-PBS cible ABL1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ABL1 Protéine recombinante Ag26152
• Nom complet: c-abl oncogene 1, receptor tyrosine kinase
• Masse moléculaire calculée: 123 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_005157
• Symbole du gène: ABL1
• Identification du gène (NCBI): 25
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ABL1, also named as ABL, JTK7, c-ABL and p150, belongs to the protein kinase superfamily, Tyr protein kinase family and ABL subfamily. ABL1 regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. ABL1 localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. It regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. ABL1 catalyze the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The antibody is specific to ABL1.

{{ptg:RelatedPrimaryAntibodies}}