Anticorps Polyclonal de lapin anti-ACAA1

ACAA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (2)

N° de cat : 12319-2-AP

Synonymes

ACAA, ACAA1, Acetyl CoA acyltransferase, Beta ketothiolase, PTHIO, THIO

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Galerie de données de validation

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  WB analysis of L02 using 12319-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12319-2-AP (ACAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12319-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12319-2-AP (ACAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12319-2-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12319-2-AP (ACAA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human thyroid cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12319-2-AP (ACAA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12319-2-AP (ACAA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12319-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12319-2-AP (ACAA1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of U-251 using 12319-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 12319-2-AP (ACAA1 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules A549, tissu pulmonaire humain
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

12319-2-AP cible ACAA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACAA1 Protéine recombinante Ag2972
• Nom complet: acetyl-Coenzyme A acyltransferase 1
• Masse moléculaire calculée: 424 aa, 44 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC011977
• Symbole du gène: ACAA1
• Identification du gène (NCBI): 30
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).

Publications

Species	Application	Title
human	IF	Hum Mol Genet; Functional analysis of PEX13 mutation in a Zellweger syndrome spectrum patient reveals novel homooligomerization of PEX13 and its role in human peroxisome biogenesis.; Authors - Krause Cindy C
human	WB	Cell Mol Life Sci; Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis.; Authors - Pablo Ranea-Robles
rat	WB	EBioMedicine; PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.; Authors - Zhongshi Lyu
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
	IF	J Agric Food Chem; Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation.; Authors - Chaochao Luo
human	IF	Sci Rep; Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes.; Authors - Kareem Soliman

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

PAULA (Verified Customer) (07-12-2023)

I tested this ACAA1 antibody on THP1 cells (20 ug total cell lysate) and two nice bands were observed at the most concentrated dilution (1:250), although they were located slightly below the predicted molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500, 1:250
• Cell Tissue Type: THP1 cell line

ACAA1 Antibody Western Blot validation (1:500, 1:250 dilution) in THP1 cell line (Cat no:12319-2-AP)

FH

Tom (Verified Customer) (04-01-2022)

1:1000 for 18 hours at 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cos7

ACAA1 Antibody Western Blot validation (1:1000 dilution) in Cos7 (Cat no:12319-2-AP)