Anticorps Polyclonal de lapin anti-ACADM

• Phare
• Validé par KD/KO

ACADM Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55210-1-PBS

Synonymes

ACAD, ACAD 1, ACAD1, EC:1.3.8.7, MCAD

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Galerie de données de validation

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  WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 55210-1-AP (same clone as 55210-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 55210-1-AP (same clone as 55210-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 55210-1-AP (same clone as 55210-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 55210-1-AP (same clone as 55210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55210-1-AP (same clone as 55210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55210-1-PBS cible ACADM dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: NM_000016
• Symbole du gène: ACADM
• Identification du gène (NCBI): 34
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

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