Anticorps Monoclonal anti-ACADM

ACADM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11G6

N° de cat : 67742-1-PBS

Synonymes

1A11G6, ACAD, ACAD 1, ACAD1, EC:1.3.8.7

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Galerie de données de validation

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  WB analysis using 67742-1-Ig (same clone as 67742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67742-1-Ig (same clone as 67742-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67742-1-Ig (same clone as 67742-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67742-1-Ig (same clone as 67742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ACADM antibody (67742-1-Ig, Clone: 1A11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67742-1-PBS cible ACADM dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACADM Protéine recombinante Ag30620
• Nom complet: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Masse moléculaire calculée: 421 aa, 47 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC005377
• Symbole du gène: ACADM
• Identification du gène (NCBI): 34
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

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