Anticorps Polyclonal de lapin anti-ACADS

• Phare
• Validé par KD/KO

ACADS Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 16623-1-AP

Synonymes

ACAD3, ACADS, Butyryl CoA dehydrogenase, SCAD

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Galerie de données de validation

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  WB analysis using 16623-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 16623-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16623-1-AP (ACADS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16623-1-AP

  IP Result of anti-ACADS (IP:16623-1-AP, 4ug; Detection:16623-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16623-1-AP (ACADS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16623-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16623-1-AP (ACADS antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules COS-7, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

16623-1-AP cible ACADS dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACADS Protéine recombinante Ag9922
• Nom complet: acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
• Masse moléculaire calculée: 412 aa, 44 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC025963
• Symbole du gène: ACADS
• Identification du gène (NCBI): 35
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human	WB	Cell Rep; HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.; Authors - De Huang
human	WB	Aging (Albany NY); ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.; Authors - Diyu Chen ; KD Validated
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Dietary iron overload mitigates atherosclerosis in high-fat diet-fed apolipoprotein E knockout mice: Role of dysregulated hepatic fatty acid metabolism.; Authors - Lin Xiao