Anticorps Polyclonal de lapin anti-ACADVL

• Phare
• Validé par KD/KO

ACADVL Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 14527-1-AP

Synonymes

ACAD6, ACADVL, LCACD, VLCAD

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Galerie de données de validation

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  WB analysis using 14527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14527-1-AP (ACADVL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14527-1-AP

  WB result of ACADVL antibody (14527-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACADVL transfected HeLa cells.

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  WB analysis using 14527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14527-1-AP (ACADVL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14527-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14527-1-AP (ACADVL antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14527-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14527-1-AP (ACADVL antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules L02, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

14527-1-AP cible ACADVL dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACADVL Protéine recombinante Ag6016
• Nom complet: acyl-Coenzyme A dehydrogenase, very long chain
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 66-73 kDa
• Numéro d’acquisition GenBank: BC012912
• Symbole du gène: ACADVL
• Identification du gène (NCBI): 37
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Very long-chain acyl-CoA dehydrogenase (VLCAD) is one of four flavoproteins which catalyze the initial step of the mitochondrial b-oxidation spiral. It belongs to the acyl-CoA dehydrogenase family and is a homodimer of a 71-kDa polypeptide which contains 2 mol of FAD/mol of enzyme. The molecular mass of the nondenatured trypsinized VLCAD is 98 kDa, by gel filtration chromatography, indicating that it is a homodimer of the 48 kDa(tryptic digest) polypeptide(PMID:9461620). Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD). It has 2 isoforms(70 kDa and 68 kDa ) produced by alternative splicing and a transit peptide. This antibody is speicifc to ACADVL.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang
human	WB	Cell Rep; HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.; Authors - De Huang
mouse	WB	Front Immunol; Impaired Autophagy and Defective T Cell Homeostasis in Mice with T Cell-Specific Deletion of Receptor for Activated C Kinase 1.; Authors - Guihua Qiu
rat	WB	Biochim Biophys Acta; Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: therapeutic effects of ginsenoside Rb1.; Authors - Jia Li
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
rat	WB	Biochim Biophys Acta; Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function.; Authors - Qian Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by ACADVL antibody (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

ACADVL Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14527-1-AP)