Anticorps Monoclonal anti-ACAP1

• Phare
• Validé par KD/KO

ACAP1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B4C9

N° de cat : 66596-1-PBS

Synonymes

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

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Galerie de données de validation

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  WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

  WB result of ACAP1 antibody (66596-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 66596-1-Ig (same clone as 66596-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66596-1-Ig (same clone as 66596-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66596-1-Ig (ACAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66596-1-PBS cible ACAP1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACAP1 Protéine recombinante Ag14254
• Nom complet: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
• Masse moléculaire calculée: 740 aa, 82 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC018543
• Symbole du gène: ACAP1
• Identification du gène (NCBI): 9744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

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