Anticorps Polyclonal de lapin anti-ACAP3

• Phare
• Validé par KD/KO

ACAP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 17570-1-AP

Synonymes

KIAA1716, Cnt-b5, CENTB5, Centaurin-beta-5, Centaurin beta 5

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 17570-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17570-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 17570-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 17570-1-AP (ACAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

17570-1-AP cible ACAP3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACAP3 Protéine recombinante Ag11777
• Nom complet: ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
• Masse moléculaire calculée: 834 aa, 92 kDa
• Poids moléculaire observé: 85kda, 92 kDa
• Numéro d’acquisition GenBank: BC047001
• Symbole du gène: ACAP3
• Identification du gène (NCBI): 116983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACAP3 (Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3) , also known as CENTB5, is a protein-coding gene that belongs to the ACAP family of GTPase-activating proteins (GAPs). ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer, ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Knockdown of ACAP3 in the developing cortical neurons of mice in utero significantly abrogated neuronal migration in the cortical layer (PMID: 28919417),

Publications

Species	Application	Title
human,mouse	WB,IF	Biochem J; ACAP3 regulates neurite outgrowth through its GAP activity specific to Arf6 in mouse hippocampal neurons.; Authors - Yuki Miura
mouse	WB	Biochem Biophys Res Commun; ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex.; Authors - Yuki Miura ; KD Validated
mouse	WB	Autophagy; Aging Differentially Affects Axonal Autophagosome Formation and Maturation; Authors - Heather Tsong

{{ptg:RelatedPrimaryAntibodies}}