Anticorps Polyclonal de lapin anti-ACAT2

• Phare
• Validé par KD/KO

ACAT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 14755-1-AP

Synonymes

acetoacetyl CoA thiolase, Acetyl-CoA acetyltransferase, cytosolic, Acetyl-CoA transferase-like protein, ACTL, Cytosolic acetoacetyl-CoA thiolase

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Galerie de données de validation

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  WB analysis of HepG2 using 14755-1-AP

  WB result of ACAT2 antibody (14755-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells.

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  WB analysis using 14755-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14755-1-AP (ACAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14755-1-AP

  IP result of anti-ACAT2 (IP:14755-1-AP, 4ug; Detection:14755-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 14755-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14755-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (14755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of HepG2 using 14755-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ACAT2 Polyclonal antibody (14755-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HepG2, cellules K-562, cellules MOLT-4, foie de rat, foie de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu hépatique humain, tissu placentaire humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 3 publications below

Informations sur le produit

14755-1-AP cible ACAT2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACAT2 Protéine recombinante Ag6503
• Nom complet: acetyl-Coenzyme A acetyltransferase 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC000408
• Symbole du gène: ACAT2
• Identification du gène (NCBI): 39
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
		Cell Res; Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.; Authors - Yanjing Guo
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
mouse	WB	iScience; Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.; Authors - George D Dalton
mouse	WB	J Agric Food Chem; Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice.; Authors - Yunxia Yang
human	WB	Food Res Int; Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo.; Authors - Suwen Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (03-01-2023)

Very good working on PVDF membrane, blocking solution milk 5%

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human pulmonary artery smooth muscle cells

ACAT2 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary artery smooth muscle cells (Cat no:14755-1-AP)

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