Anticorps Monoclonal anti-ACAT2

• Phare
• Validé par KD/KO

ACAT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F5F11

N° de cat : 68005-1-PBS

Synonymes

ACAT2, ACTL

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Galerie de données de validation

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  WB analysis using 68005-1-Ig (same clone as 68005-1-PBS)

  Various cell lysates were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68005-1-Ig (same clone as 68005-1-PBS)

  WB result of ACAT2 antibody (68005-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 68005-1-Ig (same clone as 68005-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  WB analysis using 68005-1-Ig (same clone as 68005-1-PBS)

  Brain tissues were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68005-1-Ig (same clone as 68005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68005-1-Ig (ACAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68005-1-Ig (same clone as 68005-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (68005-1-Ig, Clone: 1F5F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68005-1-PBS cible ACAT2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACAT2 Protéine recombinante Ag6596
• Nom complet: acetyl-Coenzyme A acetyltransferase 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC000408
• Symbole du gène: ACAT2
• Identification du gène (NCBI): 39
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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