Anticorps Recombinant de lapin anti-ACC1

ACC1 Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

2I13

N° de cat : 81656-1-RR

Synonymes

ACACA, ACC-alpha, ACCA, ACC, ACAC

---

Galerie de données de validation

• 

  WB analysis using 81656-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81656-1-RR (ACC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 81656-1-RR

  IP result of anti-ACC1 (IP:81656-1-RR, 4ug; Detection:81656-1-RR 1:2000) with HepG2 cells lysate 1640 ug.

• 

  Affinity and Kinetic Characterization of 81656-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81656-1-RR against Human ACC1 were performed. The affinity constant is 2.28 nM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IP: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81656-1-RR cible ACC1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACC1 Protéine recombinante Ag16452
• Nom complet: acetyl-Coenzyme A carboxylase alpha
• Masse moléculaire calculée: 2383 aa, 275 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC137287
• Symbole du gène: Acetyl-CoA Carboxylase 1
• Identification du gène (NCBI): 31
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

{{ptg:RelatedPrimaryAntibodies}}