Anticorps Monoclonal anti-ACC1

• Phare
• Validé par KD/KO

ACC1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A11G10

N° de cat : 67373-1-PBS

Synonymes

ACACA, ACC-alpha, ACCA, ACC, ACAC

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Galerie de données de validation

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  WB analysis using 67373-1-Ig (same clone as 67373-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67373-1-Ig (ACC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67373-1-Ig (same clone as 67373-1-PBS)

  WB result of ACC1 antibody (67373-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACC1 transfected HeLa cells. This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67373-1-Ig (same clone as 67373-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67373-1-Ig (ACC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67373-1-Ig (same clone as 67373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67373-1-Ig (same clone as 67373-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67373-1-Ig (same clone as 67373-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ACC1 antibody (67373-1-Ig, Clone: 1A11G10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67373-1-PBS cible ACC1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACC1 Protéine recombinante Ag17503
• Nom complet: acetyl-Coenzyme A carboxylase alpha
• Masse moléculaire calculée: 2383 aa, 275 kDa
• Poids moléculaire observé: 250-270 kDa
• Numéro d’acquisition GenBank: BC137287
• Symbole du gène: Acetyl-CoA Carboxylase 1
• Identification du gène (NCBI): 31
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

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