Anticorps Recombinant de lapin anti-ACC1

ACC1 Recombinant Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2I13

N° de cat : 81656-1-PBS

Synonymes

ACACA, ACC-alpha, ACCA, ACC, ACAC

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Galerie de données de validation

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  WB analysis using 81656-1-RR (same clone as 81656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81656-1-RR (ACC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81656-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 81656-1-RR (same clone as 81656-1-PBS)

  IP result of anti-ACC1 (IP:81656-1-RR, 4ug; Detection:81656-1-RR 1:2000) with HepG2 cells lysate 1640 ug. This data was developed using the same antibody clone with 81656-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81656-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81656-1-RR against Human ACC1 were performed. The affinity constant is 2.28 nM.

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Informations sur le produit

81656-1-PBS cible ACC1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACC1 Protéine recombinante Ag16452
• Nom complet: acetyl-Coenzyme A carboxylase alpha
• Masse moléculaire calculée: 2383 aa, 275 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC137287
• Symbole du gène: Acetyl-CoA Carboxylase 1
• Identification du gène (NCBI): 31
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

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