Anticorps Polyclonal de lapin anti-ACE

ACE Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24743-1-PBS

Synonymes

DCP1, DCP, Angiotensin-converting enzyme, soluble form, Angiotensin-converting enzyme, Angiotensin converting enzyme

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Galerie de données de validation

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  WB analysis of mouse heart using 24743-1-AP (same clone as 24743-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

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  WB analysis of rat lung using 24743-1-AP (same clone as 24743-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

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  IHC staining of rat lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 24743-1-AP (same clone as 24743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24743-1-PBS cible ACE dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACE Protéine recombinante Ag20654
• Nom complet: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• Masse moléculaire calculée: 150 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC036375
• Symbole du gène: ACE
• Identification du gène (NCBI): 1636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).

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