Anticorps Recombinant de lapin anti-ACE2

ACE2 Recombinant Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4H12

N° de cat : 80063-1-PBS

Synonymes

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

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Galerie de données de validation

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  IHC staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80063-1-RR (ACE2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80063-1-RR (ACE2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 80063-1-RR (same clone as 80063-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (80063-1-RR, Clone: 4H12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80063-1-PBS in a different storage buffer formulation.

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Informations sur le produit

80063-1-PBS cible ACE2 dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACE2 Protéine recombinante Ag30683
• Nom complet: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Masse moléculaire calculée: 805 aa, 92 kDa
• Numéro d’acquisition GenBank: BC048094
• Symbole du gène: ACE2
• Identification du gène (NCBI): 59272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088). 80063-1-RR is specific for ACE2, and its antigen region is 30-356aa.

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