Anticorps Polyclonal de lapin anti-ACIN1

ACIN1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23937-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 23937-1-AP (same clone as 23937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23937-1-AP (ACIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23937-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 23937-1-AP (same clone as 23937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23937-1-AP (ACIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23937-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23937-1-PBS cible ACIN1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACIN1 Protéine recombinante Ag21036
• Nom complet: apoptotic chromatin condensation inducer 1
• Masse moléculaire calculée: 1341 aa, 152 kDa
• Poids moléculaire observé: 250 kDa, 80-85 kDa
• Numéro d’acquisition GenBank: BC140805
• Symbole du gène: ACIN1
• Identification du gène (NCBI): 22985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. ACIN1 is a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. There are three isoforms of human acinus (the L, S and S' forms) shows at about 80-85 kDa and 220-250 kDa by Western Blot, which were probably generated by alternative splicing (PMID: 10490026). 23937-1-AP can detect all three isoforms of ACIN1. Another antibody 30522-1-AP specificly targets at the ACIN1-L.

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