Anticorps Polyclonal de lapin anti-ACIN1-L specific

ACIN1-L specific Polyclonal Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30522-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30522-1-AP (same clone as 30522-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30522-1-AP (ACIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30522-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 30522-1-AP (same clone as 30522-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ACIN1-L specific antibody (30522-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30522-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30522-1-PBS cible ACIN1-L specific dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACIN1-L specific Protéine recombinante Ag30506
• Nom complet: apoptotic chromatin condensation inducer 1
• Masse moléculaire calculée: 1341 aa, 152 kDa
• Poids moléculaire observé: 240 kDa
• Numéro d’acquisition GenBank: BC140805
• Symbole du gène: ACIN1
• Identification du gène (NCBI): 22985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. ACIN1 is a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. There are three isoforms of human acinus (the L, S and S' forms) shows at about 80-85 kDa and 220-250 kDa by Western Blot, which were probably generated by alternative splicing (PMID: 10490026). 30522-1-AP specificly targets at the ACIN1-L, while another antibody 23937-1-AP can detect all three isoforms of ACIN1.

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