Anticorps Monoclonal anti-ACLY

• Phare
• Validé par KD/KO

ACLY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E10D5

N° de cat : 67166-1-PBS

Synonymes

ATP citrate lyase, 1E10D5, ACL, ATP-citrate, ATP-citrate synthase

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Galerie de données de validation

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  WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67166-1-Ig (same clone as 67166-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67166-1-Ig (same clone as 67166-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67166-1-Ig (same clone as 67166-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67166-1-Ig (same clone as 67166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67166-1-Ig (same clone as 67166-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACLY antibody (67166-1-Ig, Clone: 1E10D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67166-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67166-1-PBS cible ACLY dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACLY Protéine recombinante Ag7709
• Nom complet: ATP citrate lyase
• Masse moléculaire calculée: 121 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC006195
• Symbole du gène: ACLY
• Identification du gène (NCBI): 47
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

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