Anticorps Recombinant de lapin anti-ACMSD

ACMSD Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241077D5

N° de cat : 84100-3-RR

Synonymes

EC:4.1.1.45, 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, 241077D5

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Galerie de données de validation

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  WB analysis of HEK-293 using 84100-3-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84100-3-RR (ACMSD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 84100-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACMSD Recombinant antibody (84100-3-RR, Clone:241077D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84100-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84100-3-RR against Human ACMSD were performed. The affinity constant is 3.49 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84100-3-RR cible ACMSD dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACMSD Protéine recombinante Ag25212
• Nom complet: aminocarboxymuconate semialdehyde decarboxylase
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC016018
• Symbole du gène: ACMSD
• Identification du gène (NCBI): 130013
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACMSD (alpha-amino-beta-carboxy-muconate-semialdehyde decarboxylase ) is the key enzyme that regulates the de novo NAD+ synthesis from tryptophan. ACMSD is highly expressed in the liver, kidney and to a lower degree in brain under physiological conditions. The enzyme is primarily cytosolic and is positioned at a critical branching point in the kynurenine pathway, wherein it influences the fate of the precursor 2-amino-3-carboxymuconic-6-semialdehyde (ACMS).

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