Anticorps Monoclonal anti-Aconitase 2

Aconitase 2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F1G4

N° de cat : 67509-1-PBS

Synonymes

ACO2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2

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Galerie de données de validation

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  WB analysis using 67509-1-Ig (same clone as 67509-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67509-1-Ig (same clone as 67509-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67509-1-Ig (same clone as 67509-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67509-1-Ig (same clone as 67509-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 67509-1-Ig (same clone as 67509-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67509-1-Ig (same clone as 67509-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67509-1-Ig (same clone as 67509-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67509-1-Ig (same clone as 67509-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67509-1-Ig (same clone as 67509-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACO2 (67509-1-Ig, Clone:1F1G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67509-1-PBS cible Aconitase 2 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Aconitase 2 Protéine recombinante Ag17784
• Nom complet: aconitase 2, mitochondrial
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC014092
• Symbole du gène: ACO2
• Identification du gène (NCBI): 50
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

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