Anticorps Polyclonal de lapin anti-ACOT11

ACOT11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10776-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 10776-1-AP (same clone as 10776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10776-1-AP (ACOT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10776-1-AP (same clone as 10776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10776-1-AP (ACOT11 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 10776-1-AP (same clone as 10776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10776-1-AP (ACOT11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 10776-1-AP (same clone as 10776-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10776-1-AP (ACOT11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 10776-1-AP (same clone as 10776-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ACOT11 antibody (10776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10776-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

10776-1-PBS cible ACOT11 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACOT11 Protéine recombinante Ag1176
• Nom complet: acyl-CoA thioesterase 11
• Masse moléculaire calculée: 68.4 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC001517
• Symbole du gène: ACOT11
• Identification du gène (NCBI): 26027
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACOT11(Acyl-coenzyme A thioesterase 11) is also named as BFIT, KIAA0707, THEA.BFIT is mapped to syntenic regions of chromosomes 1 (human) and 4 (mouse) associated with body fatness and diet-induced obesity, potentially linking a deficit of BFIT activity with exacerbation of these traits and supports the transition of this tissue towards increased metabolic activity, probably through alteration of intracellular fatty acyl-CoA concentration(PMID:11696000).

{{ptg:RelatedPrimaryAntibodies}}