Anticorps Polyclonal de lapin anti-ACOT12

ACOT12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 17871-1-PBS

Synonymes

Acetyl-coenzyme A thioesterase, ACOT 12, Acyl-CoA thioester hydrolase 12, Acyl-CoA thioesterase 12, Acyl-coenzyme A thioesterase 12

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Galerie de données de validation

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  WB analysis using 17871-1-AP (same clone as 17871-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17871-1-AP (ACOT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17871-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 17871-1-AP (same clone as 17871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17871-1-AP (ACOT12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17871-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 17871-1-AP (same clone as 17871-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ACOT12 antibody (17871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17871-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17871-1-PBS cible ACOT12 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACOT12 Protéine recombinante Ag12355
• Nom complet: acyl-CoA thioesterase 12
• Masse moléculaire calculée: 555 aa, 62 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC075011
• Symbole du gène: ACOT12
• Identification du gène (NCBI): 134526
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACOT12 is an acyl-coenzyme A thioesterase that hydrolyzes acyl-coenzyme A (acyl-CoA) to free fatty acids and coenzyme A, thereby regulating intracellular levels of acyl-CoA and free fatty acids. It plays a key role in lipid metabolism and is mainly expressed in the kidney, liver, and intestine. ACOT12 plays an important role in a variety of diseases, including renal fibrosis, nonalcoholic fatty liver disease (NAFLD), and hepatocellular carcinoma. In renal fibrosis, downregulation of ACOT12 expression leads to lipid accumulation and exacerbates the fibrotic process. Restoration of its expression ameliorates renal fibrosis, suggesting that ACOT12 is a potential therapeutic target.

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