Anticorps Polyclonal de lapin anti-ACOT4

ACOT4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17316-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of L02 using 17316-1-AP (same clone as 17316-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

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  WB analysis using 17316-1-AP (same clone as 17316-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 17316-1-AP (same clone as 17316-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOT4 antibody (17316-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17316-1-PBS cible ACOT4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACOT4 Protéine recombinante Ag11286
• Nom complet: acyl-CoA thioesterase 4
• Masse moléculaire calculée: 421 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC031799
• Symbole du gène: ACOT4
• Identification du gène (NCBI): 122970
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ACOT4, also known as PET2B and PTEIB, is an acyl-CoA thioesterases which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), regulating their respective intracellular levels. ACOT4 has the strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle(PMID: 16940157).

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