Anticorps Polyclonal de lapin anti-ACP2

ACP2 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 15236-1-AP

Synonymes

acid phosphatase 2, lysosomal, ACP2, LAP, Lysosomal acid phosphatase

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Galerie de données de validation

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  WB analysis of mouse kidney using 15236-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15236-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15236-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15236-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15236-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15236-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15236-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15236-1-AP

  Immunofluorescent analysis of HepG2 cells using 15236-1-AP (ACP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HepG2, cellules HL-60, tissu cérébral de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15236-1-AP cible ACP2 dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACP2 Protéine recombinante Ag7356
• Nom complet: acid phosphatase 2, lysosomal
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 63 kDa, 52 kDa
• Numéro d’acquisition GenBank: BC003160
• Symbole du gène: ACP2
• Identification du gène (NCBI): 53
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.